{"id":2899,"date":"2024-08-31T23:39:17","date_gmt":"2024-08-31T21:39:17","guid":{"rendered":"https:\/\/dbis.ibp.cz\/?p=2899"},"modified":"2024-09-04T21:52:43","modified_gmt":"2024-09-04T19:52:43","slug":"new-publication-growth-phase-matters-boosting-immunity-via-lacticasebacillus-derived-membrane-vesicles-and-their-interactions-with-tlr2-pathways","status":"publish","type":"post","link":"https:\/\/dbis.ibp.cz\/index.php\/2024\/08\/31\/new-publication-growth-phase-matters-boosting-immunity-via-lacticasebacillus-derived-membrane-vesicles-and-their-interactions-with-tlr2-pathways\/","title":{"rendered":"New publication: Growth Phase Matters: Boosting immunity via Lacticasebacillus-derived membrane vesicles and their interactions with TLR2 pathways"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2899\" class=\"elementor elementor-2899\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4d39720 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4d39720\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-c019e84\" data-id=\"c019e84\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-ed3afec elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"ed3afec\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-dfb759e\" data-id=\"dfb759e\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f538fb8 elementor-widget elementor-widget-heading\" data-id=\"f538fb8\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">New publication: Growth Phase Matters: Boosting immunity via Lacticasebacillus-derived membrane vesicles and their interactions with TLR2 pathways<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-09c4f04 elementor-widget elementor-widget-text-editor\" data-id=\"09c4f04\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>The teams of Dr. Ambro\u017eov\u00e1 and Dr. Turkov\u00e1 have published a new paper.<\/p><p>This study examines the properties of membrane vesicles (MVs) produced by <em>Lacticaseibacillus rhamnosus<\/em> CCM7091 at different growth stages: early exponential (6 hours), late exponential (12 hours), and late stationary phase (48 hours). Significant differences in protein content were found between MVs from the early and late stages, with the late stationary phase MVs (MV48) showing the most potent immune-stimulating effects. MV48s were more readily taken up by epithelial cells and triggered a stronger immune response in murine macrophages, likely due to increased lipoteichoic acid (LTA) expression and enhanced TLR2 signaling. These findings suggest that MVs from the late stationary phase could be particularly effective for therapeutic applications as nanocarriers.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-d230445 elementor-button-success elementor-align-center elementor-widget elementor-widget-button\" data-id=\"d230445\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-lg\" href=\"https:\/\/isevjournals.onlinelibrary.wiley.com\/doi\/10.1002\/jex2.169\" target=\"_blank\" rel=\"noopener\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Full text of the article<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-962fd29 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"962fd29\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-c75e0f0\" data-id=\"c75e0f0\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-981c293 elementor-widget elementor-widget-image\" data-id=\"981c293\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"768\" height=\"538\" src=\"https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2024\/08\/Sandanusova_Turkova_GAfigure-768x538.png\" class=\"attachment-medium_large size-medium_large wp-image-2909\" alt=\"\" srcset=\"https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2024\/08\/Sandanusova_Turkova_GAfigure-768x538.png 768w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2024\/08\/Sandanusova_Turkova_GAfigure-300x210.png 300w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2024\/08\/Sandanusova_Turkova_GAfigure-1024x717.png 1024w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2024\/08\/Sandanusova_Turkova_GAfigure-1536x1075.png 1536w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2024\/08\/Sandanusova_Turkova_GAfigure-2048x1434.png 2048w\" sizes=\"(max-width: 768px) 100vw, 768px\" title=\"\">\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Graphical abstract: Graphical Abstract Membrane vesicles (MVs) derived from Gram-positive bacteria are crucial for bacterial communication, but their variable characteristics present challenges for applications as nanocarriers or vaccines. This study on Lacticaseibacillus rhamnosus CCM7091-derived MVs, isolated throughout different growth phases, reveals significant protein differences, heightened uptake by Caco-2 cells and immune stimulation in RAW 264.7 macrophages. Late stationary phase MVs (MV48) stand out, showing elevated lipoteichoic acid and enhanced TLR2 signalling, suggesting their potential as effective immunoboosting nanocarriers for future therapeutic applications.<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>New publication: Growth Phase Matters: Boosting immunity via Lacticasebacillus-derived membrane vesicles and their interactions with TLR2 pathways The teams of Dr. Ambro\u017eov\u00e1 and Dr. Turkov\u00e1 have published a new paper. This study examines the properties of membrane vesicles (MVs) produced by Lacticaseibacillus rhamnosus CCM7091 at different growth stages: early exponential (6 hours), late exponential (12 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":2900,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[127],"tags":[],"class_list":["post-2899","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-new-publication","entry","has-media"],"_links":{"self":[{"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/posts\/2899","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/comments?post=2899"}],"version-history":[{"count":7,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/posts\/2899\/revisions"}],"predecessor-version":[{"id":2912,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/posts\/2899\/revisions\/2912"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/media\/2900"}],"wp:attachment":[{"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/media?parent=2899"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/categories?post=2899"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/tags?post=2899"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}