{"id":3632,"date":"2026-03-01T23:27:52","date_gmt":"2026-03-01T22:27:52","guid":{"rendered":"https:\/\/dbis.ibp.cz\/?p=3632"},"modified":"2026-04-01T23:01:51","modified_gmt":"2026-04-01T21:01:51","slug":"ph-d-defence-of-miriam-dutkova","status":"publish","type":"post","link":"https:\/\/dbis.ibp.cz\/index.php\/2026\/03\/01\/ph-d-defence-of-miriam-dutkova\/","title":{"rendered":"Ph.D. defence of Miriam Dutkov\u00e1"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"3632\" class=\"elementor elementor-3632\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b5ff995 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"b5ff995\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-d2fb24d\" data-id=\"d2fb24d\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5813636 elementor-widget elementor-widget-heading\" data-id=\"5813636\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Ph.D. defence of Miriam Dutkov\u00e1<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7820f37 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7820f37\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-c8afa83\" data-id=\"c8afa83\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d7a097b elementor-widget elementor-widget-text-editor\" data-id=\"d7a097b\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>On Friday, April 27, our colleague Mirim Dutkov\u00e1 defended her dissertation.\u00a0<\/p><p>This thesis investigates vesicle-mediated communication between the gut microbiota and host innate immune cells. It focuses on membrane vesicles (MVs) derived from the Gram-positive bacteria Lacticaseibacillus rhamnosus and Enterococcus faecium, and on extracellular vesicles (EVs) released by macrophages in response to bacterial stimulation. The work demonstrates that bacterial MVs activate macrophages in a TLR2-dependent manner and provide inflammasome-priming signals, with their immunostimulatory capacity influenced by bacterial growth phase. Stimulated macrophages subsequently release molecularly remodeled EVs with distinct protein and lipid profiles, which propagate microbial signals to na\u00efve macrophages and endothelial cells and are detectable in vivo during inflammatory conditions. Altogether, the thesis defines a bidirectional vesicle-mediated communication axis linking Gram-positive bacteria and host cells, advancing our understanding of how EVs contribute to intestinal immune homeostasis and inflammation.<\/p><div class=\"pointer-events-none h-px w-px absolute bottom-0\" aria-hidden=\"true\" data-edge=\"true\">\u00a0<\/div><div class=\"flex flex-col text-sm pb-25\"><article class=\"text-token-text-primary w-full focus:outline-none [--shadow-height:45px] has-data-writing-block:pointer-events-none has-data-writing-block:-mt-(--shadow-height) has-data-writing-block:pt-(--shadow-height) [&amp;:has([data-writing-block])&gt;*]:pointer-events-auto scroll-mt-[calc(var(--header-height)+min(200px,max(70px,20svh)))]\" dir=\"auto\" tabindex=\"-1\" data-turn-id=\"request-WEB:dc6656e8-9a11-4306-a12c-945de7989548-2\" data-testid=\"conversation-turn-6\" data-scroll-anchor=\"true\" data-turn=\"assistant\"><div class=\"text-base my-auto mx-auto pb-10 [--thread-content-margin:--spacing(4)] @w-sm\/main:[--thread-content-margin:--spacing(6)] @w-lg\/main:[--thread-content-margin:--spacing(16)] px-(--thread-content-margin)\"><div class=\"[--thread-content-max-width:40rem] @w-lg\/main:[--thread-content-max-width:48rem] mx-auto max-w-(--thread-content-max-width) flex-1 group\/turn-messages focus-visible:outline-hidden relative flex w-full min-w-0 flex-col agent-turn\" tabindex=\"-1\"><div class=\"flex max-w-full flex-col grow\"><div class=\"min-h-8 text-message relative flex w-full flex-col items-end gap-2 text-start break-words whitespace-normal [.text-message+&amp;]:mt-1\" dir=\"auto\" data-message-author-role=\"assistant\" data-message-id=\"fc78854b-a51d-4175-9608-7d024dd32fba\" data-message-model-slug=\"gpt-5-2\"><div class=\"flex w-full flex-col gap-1 empty:hidden first:pt-[1px]\"><div class=\"markdown prose dark:prose-invert w-full wrap-break-word light markdown-new-styling\"><p data-start=\"1059\" data-end=\"1139\" data-is-last-node=\"\" data-is-only-node=\"\">Congratulations on a rigorous and impactful piece of work successfully defended.<\/p><\/div><\/div><\/div><\/div><div class=\"z-0 flex min-h-[46px] justify-start\">\u00a0<\/div><div class=\"mt-3 w-full empty:hidden\"><div class=\"text-center\">\u00a0<\/div><\/div><\/div><\/div><\/article><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-fc0220e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fc0220e\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-5236528\" data-id=\"5236528\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4e0c012 elementor-widget elementor-widget-image\" data-id=\"4e0c012\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1024\" height=\"683\" src=\"https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/DSC_0392-Velkyx-1024x683.jpg\" class=\"attachment-large size-large wp-image-3635\" alt=\"\" srcset=\"https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/DSC_0392-Velkyx-1024x683.jpg 1024w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/DSC_0392-Velkyx-300x200.jpg 300w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/DSC_0392-Velkyx-768x512.jpg 768w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/DSC_0392-Velkyx-1536x1025.jpg 1536w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/DSC_0392-Velkyx.jpg 1619w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" title=\"\">\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-2eb8921\" data-id=\"2eb8921\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-37f9601 elementor-widget elementor-widget-image\" data-id=\"37f9601\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1024\" height=\"683\" src=\"https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/DSC_0399-Velkyx-1024x683.jpg\" class=\"attachment-large size-large wp-image-3636\" alt=\"\" srcset=\"https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/DSC_0399-Velkyx-1024x683.jpg 1024w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/DSC_0399-Velkyx-300x200.jpg 300w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/DSC_0399-Velkyx-768x513.jpg 768w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/DSC_0399-Velkyx.jpg 1254w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" title=\"\">\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-c9a923e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c9a923e\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-81b135a\" data-id=\"81b135a\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c69e2e7 elementor-widget elementor-widget-image\" data-id=\"c69e2e7\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"683\" height=\"1024\" src=\"https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112804xxx-683x1024.jpg\" class=\"attachment-large size-large wp-image-3637\" alt=\"\" srcset=\"https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112804xxx-683x1024.jpg 683w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112804xxx-200x300.jpg 200w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112804xxx-768x1151.jpg 768w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112804xxx-1025x1536.jpg 1025w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112804xxx-1366x2048.jpg 1366w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112804xxx.jpg 1601w\" sizes=\"(max-width: 683px) 100vw, 683px\" title=\"\">\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-814bf28\" data-id=\"814bf28\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-cc501ab elementor-widget elementor-widget-image\" data-id=\"cc501ab\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" width=\"683\" height=\"1024\" src=\"https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112942x-683x1024.jpg\" class=\"attachment-large size-large wp-image-3634\" alt=\"\" srcset=\"https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112942x-683x1024.jpg 683w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112942x-200x300.jpg 200w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112942x-768x1151.jpg 768w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112942x-1025x1536.jpg 1025w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112942x-1366x2048.jpg 1366w, https:\/\/dbis.ibp.cz\/wp-content\/uploads\/2026\/03\/20260227_112942x-scaled.jpg 1708w\" sizes=\"(max-width: 683px) 100vw, 683px\" title=\"\">\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Ph.D. defence of Miriam Dutkov\u00e1 On Friday, April 27, our colleague Mirim Dutkov\u00e1 defended her dissertation.\u00a0 This thesis investigates vesicle-mediated communication between the gut microbiota and host innate immune cells. It focuses on membrane vesicles (MVs) derived from the Gram-positive bacteria Lacticaseibacillus rhamnosus and Enterococcus faecium, and on extracellular vesicles (EVs) released by macrophages in [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":2614,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-3632","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-nezarazene","entry","has-media"],"_links":{"self":[{"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/posts\/3632","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/comments?post=3632"}],"version-history":[{"count":3,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/posts\/3632\/revisions"}],"predecessor-version":[{"id":3640,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/posts\/3632\/revisions\/3640"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/media\/2614"}],"wp:attachment":[{"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/media?parent=3632"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/categories?post=3632"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/dbis.ibp.cz\/index.php\/wp-json\/wp\/v2\/tags?post=3632"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}