New publication: Growth Phase Matters: Boosting immunity via Lacticasebacillus-derived membrane vesicles and their interactions with TLR2 pathways

The teams of Dr. Ambrožová and Dr. Turková have published a new paper.

This study examines the properties of membrane vesicles (MVs) produced by Lacticaseibacillus rhamnosus CCM7091 at different growth stages: early exponential (6 hours), late exponential (12 hours), and late stationary phase (48 hours). Significant differences in protein content were found between MVs from the early and late stages, with the late stationary phase MVs (MV48) showing the most potent immune-stimulating effects. MV48s were more readily taken up by epithelial cells and triggered a stronger immune response in murine macrophages, likely due to increased lipoteichoic acid (LTA) expression and enhanced TLR2 signaling. These findings suggest that MVs from the late stationary phase could be particularly effective for therapeutic applications as nanocarriers.

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Sandanusova Turkova GAfigure
Graphical abstract: Graphical Abstract Membrane vesicles (MVs) derived from Gram-positive bacteria are crucial for bacterial communication, but their variable characteristics present challenges for applications as nanocarriers or vaccines. This study on Lacticaseibacillus rhamnosus CCM7091-derived MVs, isolated throughout different growth phases, reveals significant protein differences, heightened uptake by Caco-2 cells and immune stimulation in RAW 264.7 macrophages. Late stationary phase MVs (MV48) stand out, showing elevated lipoteichoic acid and enhanced TLR2 signalling, suggesting their potential as effective immunoboosting nanocarriers for future therapeutic applications.